Question: ambiguous characters are not reported in Bowtie2 alignments
gravatar for Famf
12 months ago by
United States
Famf20 wrote:

I am trying to align a set of read sequences (SNP chip array) to a reference genome. I want to get the physical positions of each SNP in each read.

My reads are in FASTA format, e.g.


I am using bowtie2 software. In each read there is an ambiguous character (IUPAC code) which are the SNPs I am interested to get their positions in the reference genome. Base on what I understood from the Bowtie2 manual I was expecting to get the following parameters in the alignment section of my output SAM file:

CIGAR = 39M and alignment score = MD:Z:7Y32
CIGAR = 39M and alignment score = MD:Z:11Y28
CIGAR = 39M and alignment score = MD:Z:4M35

Instead I got the following parameters for almost all the alignments

CIGAR = 40M and alignment score = MD:Z:40

This is the code I am using:

bowtie2 -x <bt2.idx> -f snp_chip.fa -S results.sam --no-unal

Any idea what could I am doing wrong?

alignment • 250 views
ADD COMMENTlink modified 12 months ago • written 12 months ago by Famf20
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