I cite from the Wiki page of the Encode Imputation Challenge:
We use the MACSv2 peak caller to compute the fold-enrichment and statistical significance of smoothed counts (150 bp smoothing window) of read-starts (5' ends of reads) at each position in the genome relative to the expected number of reads from a local Poisson simulated background distribution of reads at each nucleotide in the genome.
I have basic knowledge of what calling peaks means, but I find it hard to interpret the details of this short description, in particular:
- What does it mean to do the smoothing of the counts?
- What is a local Poisson simulated background? From what is is computed?
Any explanation or reference is much appreciated.