Hi, my sequecing data contains 30 extra base pairs in the 5' which also contain UMI information. First, I want to map these reads to a reference genome and then I want to stastics the UMI information. How should I set mapping defaults? Does 30 extra bps interfere the mapping process?
My UMI information is not in the start point of a reads. They are 30bp away from 5'.
Thanks for your answers.