Question: piRNA target from small RNA Ripseq
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gravatar for giu.lat82
12 months ago by
giu.lat820
giu.lat820 wrote:

Hi I am analyzing RIP Seq experiments. I downloaded several RIPSeq experiments where the authors performed immunoprecipitation for PIWIL1 and then sequenced the small RNAs part. After performed the detection of piRNAs and other classes of small RNAs i found a huge number of reads mapping on genes (mRNAs and lncRNAs). Could they be target of the identified piRNA? Or are they degradation products? Which strategy could I use to predict piRNA- target interaction using these data?

rna-seq • 249 views
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