So I am doing some RNA seq and I have a number of large library preps for the different conditions and replicates. In some samples there a huge differences in library size and I was hoping to just do a preliminary analysis where I randomly subset x amount of reads from the parent fastq, but i want to do it multiple times from the sample fastq to generate a more representative sample. I need the sampling to therefor be random and different for each subset
Is this possible?
Thanks in advance for any help