Question: How to process RMA data of gene microarry ?
6 days ago by
Laura_zz • 0
Laura_zz • 0 wrote:
I get RMA data from ArrayExpress, and it's single-channel data. I don't know much about gene chips, so I checked some information and tried to run the script provided by the limma package myself, but I don't know if it’s right.
- One step of RMA data is to run exprs(RMA data), but I can't run it, so I use log2(RMA data+1) to replace function exprs(), is that correct?
- Should RMA data processing be processed first with log2 RMA data+1, and then with lmfit in the limma package for differential expression calculation?
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