Question: MACS2 result of FDR
0
gravatar for chaudharyc61
5 months ago by
chaudharyc6110
chaudharyc6110 wrote:

Hello everyone As one of the output file of macs2 is in excel form which tells -log10(q-value) which is the FDR (False Discover Rate) I wan to ask whether the highest q-value is reliable or the lowest as every peak has its own -log10(q-value) In other words if -log10(q-value) is 10.08 of one peak is that mean that peak has high chance to be a false positive or its the other way around ?

Thanks in advance...

ADD COMMENTlink modified 5 months ago by genomax75k • written 5 months ago by chaudharyc6110

Hi chaudharyc61, I have a question for you about that values,

how did you get that p-value?

My excel file does not have any columns for the p-value and the columns are this

chr start   end length  abs_summit  pileup  #NOME?  fold_enrichment #NOME?  name

Thank you

ADD REPLYlink written 5 months ago by Morris_Chair150

While peak calling did you change the default p-value ?

ADD REPLYlink written 5 months ago by chaudharyc6110
2
gravatar for shawn.w.foley
5 months ago by
shawn.w.foley1.1k
USA
shawn.w.foley1.1k wrote:

An FDR (or q-value) is an adjusted p-value, therefore the lowest q-value is the most significant. However, in this case you're not looking at q-values, but at -log10(q-value), in which case the highest number is more informative (because of that negative sign). For example, if q = 10^-10 that's a highly significant value, and -log10(10^-10) = 10, a high positive number.

The lowest q-value is most significant, and the highest -log10(q-value) is most significant.

ADD COMMENTlink written 5 months ago by shawn.w.foley1.1k
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