Hello,
I am looking for stat expertise to figure out if I have set up my model matrix correctly. I am going to give an example info table that represents my experimental design. I have read the limma and edgeR documentation through and through and cannot find a clear example of this.
DESIGN: 3 individuals with recordings of a treatment at baseline and 3 future time points. baseline is preinfection. timepoints relate to stage in disease progression.
file individual timepoint status
file <- 1:12
individual <- c(rep(1,4),rep(2,4),rep(3,4))
timepoint <- rep(0:4,3)
status <- rep(c("uninfected",4),rep("infected",8))
df <- cbind(file,individual,timepoint,status)
I am interested in the differential expression at each timepoint with timepoint 0 being baseline.
model.matrix(~individual+timepoint)
model.matrix(timepoint~(1|individual))
The main effect I want to see is the effect between timepoints not individuals- so I understand I can treat the individuals as a random effect in a mixed model. I just am not sure not to correctly integrate multiple independent variables, paired samples, and longitudinal data with baseline measures.
Any help and or explanations would be greatly appreciated!
I suggest you post this over at Bioconductor as the maintainers of the tools are active there. If you do so, please use only one tag, either limma OR edgeR or DESeq2 depending on the tool you want to use. It is generally (at BioC, not here at Biostars) bad practice to use multiple tool tags as it will attract all developers and they already have quite a workload given all the questions they receive. If you have no preference, simply do not use a tool tag and only use something like
model matrix.This is more of a general linear regression stat question that could relate to many different packages- but I did post in BioC with only the limma tag. Thank you for the suggestion.
Cross-posted as suggested: https://support.bioconductor.org/p/122407/