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Hello to everyone. I have regularized log2- transformed count data (from bulk RNAseq), generated by DESeq2 function rlog. I have technical triplicates of cells untreated and treated. I´ve been suggested to calculate the fold change between the triplicates for every gene to see easily a difference in the gene expression between untreated and treated conditions. Due to the triplicates are quite consistent between each other for all the genes, I decided to calculate the mean first and then make the ratios of the means (treated/untreated) to calculate the fold change. My questions are: 1) Does it make sense first to calculate a fold change from regularized log2- transformed count data generated by DESeq2 function rlog? 2) Is correct to calculate in this way a fold change? Thank you all so much for the replies and help!

**7.4k**• written 7 months ago by tommaso.gastaldi •

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