Hello everyone, I'm analyzing data using the new tuxedo package (HISAT, StringTie, and Ballgown) but I have problem to make ballgown working
pf_rna<-ballgown(dataDir="ballgown/", samplePattern = sample, pData=pheno_data)
I get this error
Sat Jul 6 00:55:39 2019 Sat Jul 6 00:55:39 2019: Reading linking tables Sat Jul 6 00:55:40 2019: Reading intron data files Sat Jul 6 00:55:41 2019: Merging intron data Sat Jul 6 00:55:43 2019: Reading exon data files Sat Jul 6 00:55:45 2019: Merging exon data Sat Jul 6 00:55:47 2019: Reading transcript data files Sat Jul 6 00:55:48 2019: Merging transcript data successfully rearranged! Wrapping up the results Sat Jul 6 00:55:50 2019 Warning message: In ballgown(dataDir = "ballgown/", samplePattern = sample, pData = pheno_data) :
Rows of pData did not seem to be in the same order as the columns of the expression data. Attempting to rearrange pData...
The name of the pData have the same order of the name of the folders where the files .ctab are located but after doing different attempts to fix it I'm exhausted and I need help ... Do you have any suggestion? I appreciate