I tried to align 112626 sequences of alfalfa using muscle command, but I get segmentation fault 11 error which is mainly due to low running memory. Then I split into 8 smaller files with 14100 sequences in each file. The muscle command ran smoothly in those files and generated output. However, when I used the -profile option in muscle command to join those 8 aligned files (2 at a time) it gives me error message (* WARNING Invalid byte hex 00 in FASTA sequence data, ignored ERROR * Internal error MSA::ExpandCache, ColCount changed). Why am I getting this error message? and how can I join those 8 small files into single aligned file?
Question: (Closed) Multiple Sequence Alignment using muscle
7 months ago by
atitparajuli2018 • 0
atitparajuli2018 • 0 wrote:
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