Hi, I have my RNAseq data all annotated already and have done further analysis. I would like to cluster the genes in function or metabolic pathways. Is there a way of generating this clusters/groups with the list of gene names? I have never used KEGG or GO analysis but I think they need the reads files?
My organism is a bacteria whose genome is found in NCBI, but its not common, so it is not found in some useful softwares such as "g:Profiler".
Thanks!
You should probably add to the title of your question that you're dealing with bacteria.
I personally have no experience with the state of the annotation for bacterial genomes, but a quick google search let to this data base: https://metacyc.org/
Apart from that, if you're able to use R, clusterProfiler is probably the tool you're looking for (if your organism of interest has KEGG annotation)