Entering edit mode
4.7 years ago
Morris_Chair
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350
Hello, everyone I have a question about samples replicates of RNA sequencing depth.
When I run the heatmap in my set of experiments with DESeq2, the replicates are not always together and the position can change based on the number of genes that I consider (100 or 1000). I noticed that this effect is more evident in samples where the number of reads is very different although normalized with DESeq2. Is it possible to have variance between biological replicates also because of different sequencing depth?
Thank you