Entering edit mode
4.8 years ago
guptavipin142
•
0
Hello Folks,
My vendor gave me file for paired end data like this
@M04631:335:000000000-CDFRH:1:2106:7814:1782 1:N:0:ATGCGCAG+AAGGCTAT
CCTACGGGGGGCAGCAGTCGGGAATTTTGGTCAATGGGCGAAAGCCTGACCCAGCCACGCCGCGTGAAGGATGAATTATTTCGGTATTTTAACTTCGTATTATTGGGAAGAATAAATGACTGTACCTTTTATAAGGTCCGTCTAACTACGTTCCAGCAGCCGCGGTAATACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTAGGCGGCCTAACAAGTCACTTGTGAAATCTCCGGGCTTAACTCGGAATGGCCAAGTGAAACTGTCAGGCTAGAGTGCAGAAGGG
+
C@CCCGDB5=@1+,894C8,477+:=EE<+-:--C<,=<C::+=<BF,,,?=88E+,,+3=:>=C7+8,@8@,,,,<,38@9,*C7D93,8F,<@,*,,,33,<<<,,?>3::A?,8,,<,8@,,,,@F:;F,,,88,8<*=F*82>,2=<,<,,,<,<FGC*:8:/::<:FCFCGCF5:88/;CGEEEFGDGGGGGFFFFEFFEG8E>?7CECEEEE>8@?E88;*88<+;<:9:9:CF@>0?F0<9+)1?*/;8<<:+;9?<*0C*+92;:F@FGGCGFF<@65F*/3CE*-:<9/0>D
@M04631:335:000000000-CDFRH:1:2106:16910:1792 1:N:0:ATGCGCAG+AAGGCTAT
CCTACGGGAGGCAGCAGTAAGGAATATTGCACAATGTGCGCAAGCCTGATCCAGCCATACCGCGTGCAGGAAGAATGTCCTTTTTTTTTTAAACTCCTTTTATATGGGAAGAATGGCTTCTTCTTTTCGCATTTTGACTTTACCATACTACTAAGCACCGGCTAACTCCGTGCCATCAGCCGCGGTAATACGTAGCGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTGCTAAGATAGGTGTGAAATCACAGAGCTTAACCTGGGAACGGCGGTTGTGATTAG
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I am processing the data with qiime2 but not sure what value to be used for barcodes in metadata for each forward and reverse file. Any help will be useful
Index sequences for
R1
andR2
are present in the fastq header.e.g.
ATGCGCAG+AAGGCTAT
representR1_index+R2_index