Question: MIseq-Paired End Data Barcodes
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guptavipin142 • 0 wrote:
Hello Folks,
My vendor gave me file for paired end data like this
@M04631:335:000000000-CDFRH:1:2106:7814:1782 1:N:0:ATGCGCAG+AAGGCTAT
CCTACGGGGGGCAGCAGTCGGGAATTTTGGTCAATGGGCGAAAGCCTGACCCAGCCACGCCGCGTGAAGGATGAATTATTTCGGTATTTTAACTTCGTATTATTGGGAAGAATAAATGACTGTACCTTTTATAAGGTCCGTCTAACTACGTTCCAGCAGCCGCGGTAATACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTAGGCGGCCTAACAAGTCACTTGTGAAATCTCCGGGCTTAACTCGGAATGGCCAAGTGAAACTGTCAGGCTAGAGTGCAGAAGGG
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C@CCCGDB5=@1+,894C8,477+:=EE<+-:--C<,=<C::+=<BF,,,?=88E+,,+3=:>=C7+8,@8@,,,,<,38@9,*C7D93,8F,<@,*,,,33,<<<,,?>3::A?,8,,<,8@,,,,@F:;F,,,88,8<*=F*82>,2=<,<,,,<,<FGC*:8:/::<:FCFCGCF5:88/;CGEEEFGDGGGGGFFFFEFFEG8E>?7CECEEEE>8@?E88;*88<+;<:9:9:CF@>0?F0<9+)1?*/;8<<:+;9?<*0C*+92;:F@FGGCGFF<@65F*/3CE*-:<9/0>D
@M04631:335:000000000-CDFRH:1:2106:16910:1792 1:N:0:ATGCGCAG+AAGGCTAT
CCTACGGGAGGCAGCAGTAAGGAATATTGCACAATGTGCGCAAGCCTGATCCAGCCATACCGCGTGCAGGAAGAATGTCCTTTTTTTTTTAAACTCCTTTTATATGGGAAGAATGGCTTCTTCTTTTCGCATTTTGACTTTACCATACTACTAAGCACCGGCTAACTCCGTGCCATCAGCCGCGGTAATACGTAGCGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTGCTAAGATAGGTGTGAAATCACAGAGCTTAACCTGGGAACGGCGGTTGTGATTAG
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I am processing the data with qiime2 but not sure what value to be used for barcodes in metadata for each forward and reverse file. Any help will be useful
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modified 6 months ago
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genomax ♦ 76k
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written
6 months ago by
guptavipin142 • 0
Index sequences for
R1andR2are present in the fastq header.e.g.
ATGCGCAG+AAGGCTATrepresentR1_index+R2_index