MIseq-Paired End Data Barcodes
0
0
Entering edit mode
4.8 years ago

Hello Folks,

My vendor gave me file for paired end data like this

@M04631:335:000000000-CDFRH:1:2106:7814:1782 1:N:0:ATGCGCAG+AAGGCTAT
CCTACGGGGGGCAGCAGTCGGGAATTTTGGTCAATGGGCGAAAGCCTGACCCAGCCACGCCGCGTGAAGGATGAATTATTTCGGTATTTTAACTTCGTATTATTGGGAAGAATAAATGACTGTACCTTTTATAAGGTCCGTCTAACTACGTTCCAGCAGCCGCGGTAATACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTAGGCGGCCTAACAAGTCACTTGTGAAATCTCCGGGCTTAACTCGGAATGGCCAAGTGAAACTGTCAGGCTAGAGTGCAGAAGGG
+
C@CCCGDB5=@1+,894C8,477+:=EE<+-:--C<,=<C::+=<BF,,,?=88E+,,+3=:>=C7+8,@8@,,,,<,38@9,*C7D93,8F,<@,*,,,33,<<<,,?>3::A?,8,,<,8@,,,,@F:;F,,,88,8<*=F*82>,2=<,<,,,<,<FGC*:8:/::<:FCFCGCF5:88/;CGEEEFGDGGGGGFFFFEFFEG8E>?7CECEEEE>8@?E88;*88<+;<:9:9:CF@>0?F0<9+)1?*/;8<<:+;9?<*0C*+92;:F@FGGCGFF<@65F*/3CE*-:<9/0>D
@M04631:335:000000000-CDFRH:1:2106:16910:1792 1:N:0:ATGCGCAG+AAGGCTAT
CCTACGGGAGGCAGCAGTAAGGAATATTGCACAATGTGCGCAAGCCTGATCCAGCCATACCGCGTGCAGGAAGAATGTCCTTTTTTTTTTAAACTCCTTTTATATGGGAAGAATGGCTTCTTCTTTTCGCATTTTGACTTTACCATACTACTAAGCACCGGCTAACTCCGTGCCATCAGCCGCGGTAATACGTAGCGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTGCTAAGATAGGTGTGAAATCACAGAGCTTAACCTGGGAACGGCGGTTGTGATTAG
+

I am processing the data with qiime2 but not sure what value to be used for barcodes in metadata for each forward and reverse file. Any help will be useful

next-gen sequencing • 686 views
ADD COMMENT
0
Entering edit mode

Index sequences for R1 and R2 are present in the fastq header.

e.g. ATGCGCAG+AAGGCTAT represent R1_index+R2_index

ADD REPLY

Login before adding your answer.

Traffic: 2643 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6