Question: Predict frameshift mutation consequence tool - causing a premature stop codon downstream
gravatar for vntt2407
14 months ago by
vntt240710 wrote:

As the title said, I have a frameshift mutation identified and for it I would like to see if it causes a premature stop codon downstream.

Is there any tools available or a way to work it out manually?

Much appreciated, thanks.

sequencing tool next-gen • 436 views
ADD COMMENTlink modified 14 months ago by maxime.policarpo110 • written 14 months ago by vntt240710

I do not know how much background you have towards bioinformatics, but if you only have one mutation you can simply replace add that mutation to the sequence and load it into e.g. Expasy Translate, and then see if a stop codon comes up or not.

ADD REPLYlink written 14 months ago by ATpoint38k
gravatar for maxime.policarpo
14 months ago by
France, Paris
maxime.policarpo110 wrote:

You can import the WT gene and the gene which contain a frameshift on MEGA and translate prior to alignment to see if it brings a premature stop codon

ADD COMMENTlink written 14 months ago by maxime.policarpo110
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