Mapping quality in Oxford Nanopore
0
0
Entering edit mode
23 months ago
rah ▴ 20

Now i've seen in several post here at Biostars, that if we have mapping qualities of 0, this means the reads are multimapping.

The data im analyzing comes from Oxford Nanopore sequencing which in general have a higher sequencing error, when compared to other sequencing tools.

So my question would be, beside the multimapping would it be possible if the read have a high error rate, that the quality will be 0, which would likewise result in a low accuracy?

Thanks a lot for your time and help

Minimap2 Mapping Quality Oxford Nanopore • 982 views
ADD COMMENT
1
Entering edit mode

Even if you have many low-quality bases, it is extremely unlikely that a multi-kb long read will map to different locations equally well due to that.

ADD REPLY

Login before adding your answer.

Traffic: 2491 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6