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4.8 years ago
BabySteps
▴
10
Need some advice on my current work. I need experts to look at my perseus workflow. It's my first time to work on this. Please assist, thanks
Hey BabySteps,
I'd suggest that you read Brief Reminder On How To Ask A Good Question as it will help you formulate better questions and more informative posts on this platform.
good day and thanks for the reply and for the attention
i have 4 sets TMT-labelled mass spec raw files from paired disease-normal tissue, and 2 extra channels as internal referernce for normalization. And I have their average abundance, abundance ratio, and other quantitation from each labelled sample - 126, 127D, 127N, 128-D, 128-N, 129-D, 129-N, 130-D, 130-N, and 131
I want to quantify each protein from each patient one at a timedf, an determine those up and down-regualted. My question is how do I make use of the 2 extra channels for in doin quantitation. Thank you very much for your recommendation and time.
Babysteps
How can we help you with your workflow if you don't show it?
good day and thanks for the reply and for the attention
i have 4 sets TMT-labelled mass spec raw files from paired disease-normal tissue, and 2 extra channels as internal referernce for normalization. And I have their average abundance, abundance ratio, and other quantitation from each labelled sample - 126, 127D, 127N, 128-D, 128-N, 129-D, 129-N, 130-D, 130-N, and 131
I want to quantify each protein from each patient one at a timedf, an determine those up and down-regualted. My question is how do I make use of the 2 extra channels for in doin quantitation. Thank you very much for your time.
Babysteps