Question: (Closed) Why Pcr need for Dna sequencing?
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gravatar for 9521ljh
15 months ago by
9521ljh20
9521ljh20 wrote:

I know PCR technique need for accuracy.

But in DNA sequencing preprocessing, the eliminating duplication read is processed.

I think duplication read is come from PCR. Then why we proceed PCR??? We will anyway eliminate it.

I think i am misunderstanding something....

sequencing assembly genome • 231 views
ADD COMMENTlink written 15 months ago by 9521ljh20

Hello 9521ljh!

We believe that this post does not fit the main topic of this site.

not related to bioinformatics. If you don't have enough DNA, you need to amplify it.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink modified 15 months ago by WouterDeCoster44k • written 15 months ago by Pierre Lindenbaum131k

There are PCR free library kits available. They may not be always available for every application but you can search for them.

ADD REPLYlink modified 15 months ago • written 15 months ago by genomax91k
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