Fully funded Horizon 2020 Marie Sklodowska-Curie PhD position within the European Industrial Doctorate project STACCATO aimed at enhancing upstream biopharmaceutical manufacturing process development through single cell analysis.
The successful candidate will be primarily located at BDs research centre of excellence in Limerick, Ireland with University College Dublin acting as the PhD awarding body. As part of the collaborative STACCATO agreement, the successful candidate will have two four-month secondments with other members of the STACCATO consortium.
The project aims to develop BD Rhapsody gene panels to monitor cellular behaviour during biopharmaceutical production. The BD Rhapsody workflow incorporating BDs highly accurate molecular indexing technology enables the measurement of both protein and gene expression in the same cell. Here we will use it to monitor and elucidate pathways and biomarkers associated to sample phenotype and cellular behaviour in cell culture processes in biopharmaceutical production development. Through collaboration, we will develop and validate Rhapsody primer panels targeting pathways of interest in selected cell lines and primary cells with a view to improving their performance as biopharmaceutical factories.
Specific Eligibility Criteria on the H2020 Marie Sklodowska Curie programme:
At the time of recruitment, the applicants should be in the first four years (full-time equivalent research experience) of their research careers and have not been awarded a doctoral degree. Mobility rule: At the time of recruitment, applicants should not have resided or carried out main activity (studies, work, etc.) in the country of the host for more than 12 months in the 3 years prior to the recruitment date. Language skills: Applicants must demonstrate that their ability to understand and express themselves in both written and spoken English is sufficiently high. The STACCATO consortium is committed to increasing the percentage of female scientists and therefore especially encourages applications from potential female ESRs.
An honours BSc or MSc in biology, biotechnology, biochemistry or a related discipline that facilitates their enrolment in a PhD program at UCD.
Knowledge of molecular biology and transcriptomics and the application of these technologies in biopharmaceutical process development.
Fluency in written and spoken English.
Hands-on experience with cell line and primary cell cultures. In-depth knowledge in transcriptome analysis; experienced in flow cytometry and cell sorting.
Practical experience with mammalian cell culture and handling of mammalian cells.
Experience of working in multidisciplinary research teams.