Question: What is 'reverse' blast, and when to use it?
0
gravatar for ravila
12 months ago by
ravila0
ravila0 wrote:

I have heard about 'reverse' blast searches, such as using RPS-BLAST. According to the website,

RPS-BLAST (Reverse PSI-BLAST) searches a query sequence against a database of profiles. This is the opposite of PSI-BLAST that searches a profile against a database of sequences, hence the 'Reverse'.

I still don't understand exactly what is the meaning and purpose of this... like what would be a good use case?

blast reverse blast rps-blast • 709 views
ADD COMMENTlink modified 12 months ago by Mensur Dlakic6.0k • written 12 months ago by ravila0
1
gravatar for Mensur Dlakic
12 months ago by
Mensur Dlakic6.0k
USA
Mensur Dlakic6.0k wrote:

PSI-BLAST takes a single sequence query and searches a large sequence database (>200 million sequences). It builds a multiple alignment from your sequences, converts it into a profile, and iterates through that process. Assuming that PSI-BLAST finds more than one match for your sequence, this process can be summed up as comparing a single profile (an alignment, if you will) against a large number of individual protein sequences.

RPS-BLAST is the opposite of that. It has a pre-made database of profiles (alignments), and searches your single sequence against it. It is many orders of magnitude faster because there are less than 100 thousand profiles. While RPS-BLAST is faster, it will give you something only if the matching profile for your sequence already exists in the database, or if it can be detected with statistical significance. PSI-BLAST is slower, but generally more sensitive because it builds a position-specific (PS) profile based exactly on your query.

In a nutshell: PSI-BLAST searches a single alignment against a database of many individual sequences, while RPS-BLAST searches a single sequence against a database of many individual alignments. The former is slower but more sensitive, while the latter is faster but may not yield all (or any) matches.

ADD COMMENTlink modified 12 months ago • written 12 months ago by Mensur Dlakic6.0k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 694 users visited in the last hour