chip seq data visualization in UCSC genome browser/Chip base / cistrome browser
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4.7 years ago

Good afternoon,

My research question is " if Androgen receptor (AR) transcription factor binds to promotor region on miRNAs ( hsa-mir-17) in breast cancer".

For this I have looked in UCSC genome browser for ENCODE transcription factors chip seq data. where I see that "AR" is not listed in the transcription factors list. https://genome.ucsc.edu/cgi-bin/hgTrackUi?hgsid=742392837_HSMmyxFwmietKNRVgruVaKro6aGL&c=chr21&g=encRegTfbsClustered

For this reason, I went to search in other two chip sew databases. 1) Cistrome data browser. http://cistrome.org/db/#/

Which gave me this

http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr13%3A87150647%2D95550646&hgsid=742386221_jKKBhI58UoKIrktFgFosw1DQafgd Can any one please help me understand, what this means? This peak means does AR binds to promoter region? How can I see binding motif in this?

2)Chip base chipbase> miRNA> transcription factor AR > dataset " MDA-MB-453 breast cancer,16h DHT treatment" I found following page. http://rna.sysu.edu.cn/chipbase/regulation_locus_browse.php?organism=human&assembly=hg38&tf_symbol=AR&sample_id=HUMHG02225&stream=up-30kb&target_symbol=hsa-mir-17&ref_gene_id=MI0000071 This gives the information in Table format, I like to see this either binding motif form or peaks.Please suggest is there any way I can look for this dataset in UCSC genome browser?

Thanks so much in advance,

chipbase encode ChIP-Seq • 1.5k views
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