Hi, I have read counts from 10X scRNA Seq data from 3 samples from two different conditions. I want to infer and then compare the inferred regulatory network between the two conditions, for which I am planning to use bigSCal2.
To infer the regulatory network, I am planning to use the read counts from all the 3 samples from a condition (let's say it "A") together to compare it with the rest of the 3 samples from other condition (let's say it "B") together. I am not sure whether it would be the correct approach or should I infer the regulatory network in each sample separately.
I will really appreciate your suggestions.
Thanks
Maybe you want to try asking the authors of
bigscale2
themselves, e.g. via their github issues?Ya, I have reached them out but also looking for suggestions from the community who all are dealing with the similar research question.
Is there any one who can help me in this regard. Thanks