I have a set of time-course RNA-seq datasets.
The experiment design is as follows:
1. For each condition, there are 3 replicates. 2. In total, there are 5 time points: T0; T1; T2; T3; T4.
I notice that there is a tutorial from this website (http://master.bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html#time-course-experiments) that explains how to perform time-course analysis for a control and treatment time series.
However, in my case, there is no control-treatment. I wonder how to make the design in DESeq2 right and if there is a need to consider the replicates as a variable in the design?