I'm trying to get something done that sound super simple but I'm stuck and I don't know how to go about it.
I have a large excel file with Primer sequences that someone designed for the human genome and I need to find out which part of the genome they cover. All i have is the sequence for the forward and reverse primer. So ideally I'd like to align the forward and reverse primer to the genome, I only want perfect matches to the genome because the primers have been designed in such a way.
There should be a linux tool to do this right? Maybe Blast, or bowtie? If anyone could guide me in the right direction I would much appreciate that.