This might be a stupid question but I am new in bioinformatics.
I have few FASTA files which I want to run reciprocal blast. Can anyone please guide me through makeblastdb command line process.
- I have downloaded NCBI blast database and extracted nr FASTA file through blastdbcmd
- Should I run makeblastdb command with my FASTA file or nr FASTA to create database?
- what will be the next step after creating database?