Hello!!
When I do capture enrichment with probes, usually are captured both strands of DNA, correct? So to capture both strands I must have a pool of double strands probes? And in this case when I hybridize double strand probes with my double strand DNA, a fraction of this probes do self annealing?
Instead what about RNA probes that are single strand, how they can capture both dna strands?Usually RNA probes capture both strands of DNA? I have to create a probe for plus strand and one for minus strand? And they don't do self annealing? I Know that RNA-RNA hybrids are more stable that DNA-DNA hybrids...
And I read that RNA-DNA hybrids are more stable that DNA-DNA hybrids, so some companies prefer to use these type of probes. But why RNA-DNA are more stable?
Sorry for many questions. Thank you in advance!!
This is not a bioinformatics question and would be best posted on other forums. Biology stackexchange or SeqAnswers.com would be possibilities.