Question: Seurat FeaturePlot: highlight only cells coexpressing several genes
gravatar for yassin
6 months ago by
yassin0 wrote:

We're currently analysing some 10X single cell RNA-seq data using Seurat v3. Using FeaturePlot, I can get UMAP plots for a set of genes comparing Control and Experiment groups, with cells expressing my gene of interest being highlighted and the dot intensity indicating gene expression.

What I'd like to do now however is to generate UMAP plots for both Control and Experiment groups where only those cells are highlighted that coexpress a set of marker genes that I define, so that I show differences in a given cell population in my UMAP plot between both groups. I already found that FeaturePlot can compare two genes and their expression level using the blend attribute - but I don't need any expression level annotation, I just want all cells to be drawn and those who show coexpression (of any level) to be annotated in colour.

What would be the way to do this?

seurat single cell rna-seq • 523 views
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