At the moment, I have some small-RNA enriched sequencing data from disease samples, read length is around 20-30 bp, tRNA largely dominated the datasets.
Could you please suggest any tool(s) or tutorials for:
Extract and assemble for only miRNA or pre-miRNA
Identify and quantify miRNA against a custom build database of miRNA
Differential expression tools that is tailored for miRNA (if any)
I am totally new to this analyses, so all comments are greatly appreciated! Thank you very much in advance for your help.