Hello!, I'm having problems with Cuffdiff analysis of gene Diff. expression. The code is:
cuffdiff -o (dir_output) --library-type=fr-firststrand -b ./dir/dir/file.fa -L Mock,Treated -u ./dir/file_gene_models.gtf
and followed by the inputs (with replicates).
In the terminal, the following is printed:
*Warning: Could not connect to update server to verify current version. Please check at the Cufflinks website (http://cufflinks.cbcb.umd.edu). [11:19:22] Loading reference annotation and sequence. [11:19:23] Inspecting maps and determining fragment length distributions. Segmentation fault: 11*
As a result, I get all expected files but with NO content (0 bytes).
Has anyone encountered such problem? I have a macbookpro with 8gb RAM and close to 140gb free space.