Entering edit mode
4.6 years ago
cf556
▴
10
Hello, I am not sure if this is the best place to post this, but I figured here I have the best chance of getting an answer, currently I am trying to run ascend's runDESeq() function and am getting the following error.
runDESeq(em.set, group = "cluster", parallel = FALSE, method = "pooled-CR")
Error in `contrasts<-`(`*tmp*`, value = contr.funs[1 + isOF[nn]]) : contrasts can be applied only to factors with 2 or more levels
Now as far as I can tell I should have at least two levels as there are three clusters, I am not sure what I am doing wrong. Any advice is appreciated.
Is
cluster
a factor or just a character class vector?