Question: Salmon GC bias with caution
1
gravatar for Morris_Chair
10 months ago by
Morris_Chair190
Morris_Chair190 wrote:

Hello everyone, I'm using salmon for the alignment of SE sequencing fastq file. at some point I have this output:

[warning] Fragment GC bias correction is currently *experimental* in single-end libraries.  Please use this option with caution.

It's not clear to me what does it mean to use this parameter ( --gcBias ) with caution, what happens if I use it? can I or not?

Thank you, in particular to who made this amazing tool

rna-seq salmon • 485 views
ADD COMMENTlink modified 10 months ago by h.mon30k • written 10 months ago by Morris_Chair190
2
gravatar for h.mon
10 months ago by
h.mon30k
Brazil
h.mon30k wrote:

There is a lengthy discussion about it at Salmon issue 83:

"--gcBias" option for single-end reads?

The crux of the problem is (and I quote rob-p, Salmon developer) "Given a single-end read, one does not know the length of the fragment from which it originates". If I am not mistaken, the method implemented just uses the mean fragment length to apply the GC-bias correction, and wasn't subjected to extensive validation, thus it is considered experimental.

ADD COMMENTlink written 10 months ago by h.mon30k
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