Is it conventional/required to use a cut-off value to make rpkm values meaningful? For example, in a transcriptome analysis if a contig/gene (mRNA) has rpkm values of 0.025 and 0.0025 in two different conditions, the DE is 10. The ratio is high but the actual values (0.025 vs 0.0025) are very low. Should I consider this DE as significant (with multiple reps and p-value <.05)?
Is there a guideline to follow/decide what is meaningful and what not?