I am wondering how to index a very large fasta file (~100GB) by using bowtie2-build? I have enlarged my memory up to 512G and use the parameter "--bmax 3 --dcv 4096" to minimize the memory usage, but it still failed...
So is there any possible solution to solve this problem? Thanks~
Here is the error message:
Using parameters --bmax 3 --dcv 4096
Doing ahead-of-time memory usage test
Ran out of memory; automatically trying more memory-economical parameters.
Total time for call to driver() for forward index: 01:17:56