Question: Condition of the chromosomes during the Chip-seq experiment for histone marks.
0
gravatar for panacotaforcota
3 months ago by
Russia
panacotaforcota0 wrote:

Hello!

In which condition are chromosomes during the Chip-seq experiment for histone marks? Is it interphase or mitotic state?

chip-seq • 141 views
ADD COMMENTlink modified 3 months ago • written 3 months ago by panacotaforcota0
2
gravatar for ATpoint
3 months ago by
ATpoint26k
Germany
ATpoint26k wrote:

I would say interphase. Generally a cell should be in the interphase longer than in the mitotic phase where the chromatin is massively condensed and by this not suited for any ChIP-like experiments. A population of cells is typically never uniform in terms of cell cycle state. They are usually a heterogeneous consortium, even if being the same cell type or a cell line. The odds to catch all of them right in a mitotic stage is probably quite low. An exception would probably be if you synchronize your cells by e.g. serum starvation (all cells would have more/less the same cell cycle status at a given time point) and then you catch a time point where they are all in mitotic phase. I never heard or read that people commonly do this. An exception could be that a certain protein that you ChIP is known or under suspision to have very cell-cycle-dependent activity in terms of DNA binding, but this is thinking aloud, I do not have examples for this.

ADD COMMENTlink written 3 months ago by ATpoint26k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2120 users visited in the last hour