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4.6 years ago
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uk.ac.babraham.FastQC.Sequence.SequenceFormatException: Midline '@A00818:33:HKYV3DSXX:4:2433:19217:4366 1:N:0:AGCCAATT' didn't start with '+' at uk.ac.babraham.FastQC.Sequence.FastQFile.
I used the solution for the previous posts.I get this: gzip: invalid compressed data--format violated Is my data originally damaged?
Please consider using a concise title. Posting an error message in title is not good etiquette.
Grep that header and see what the fastq record looks like:
grep -A 3 "@A00818:33:HKYV3DSXX:4:2433:19217:4366" your_file. Third line in the fastq record should have+.I just tried it, but there is no output. then i used the zgrep I got this error:
gzip: YS1_S1_L002_R1_001.fastq.gz: invalid compressed data--format violated
It is likely that your file is corrupted. Can you obtain a new copy?
Ok, I will go to get a copy of the data, thank you.