Help with Masurca and scripts to use and how to go about

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4.7 years ago

NPB • 0

Hi All,

I am trying to work on genome assembly and plan to use minION sequencing for generating long reads for a fungus. I am very new to this realm of bioinformatics and would really appreciate it if someone could guide me on how to proceed with the data. I will be doing some Illumina sequencing as well to generate short reads which which then be used to polish the long reads. I am hoping to get some tutorials or information on scripts I should be running. I am just starting working on terminal as such and would really appreciate inputs about how to go about this. I would really like to get some help with MaSuRCA script to test the data.

Thanks

MaSuRCA Assembly genome • 928 views

ADD COMMENT • link 4.7 years ago by NPB • 0

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In your post title, you have mentioned Masurca assembler. Do you essentially need that ? Have a look at this tutorial for assembly polishing

https://github.com/nanoporetech/ont-assembly-polish

ADD REPLY • link 4.7 years ago by lakhujanivijay 5.8k

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Hi,

Thanks a lot for your reply. No, I don't essentially need to use that tool as such but it was advised to me by one of our collaborators. I will surely look at this. Thanks.

ADD REPLY • link 4.7 years ago by NPB • 0

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