Hello Guys,
I am running the QTL-seq program on anaconda3. As suggested from Github. facing the following error, Can anay one suggest how to reslove it? ~$ qtlseq -r Gmax_275_v2.0.fa \
-p RT4_filtered_1.fastq.gz,RT4_filtered_2.fastq.gz \ -b1 RT1_filtered_1.fastq.gz,RT1_filtered_2.fastq.gz \ -b2 RT2_filtered_1.fastq.gz,RT2_filtered_2.fastq.gz \ -n1 10 \ -n2 10 \ -o /home/user1/Test_dir [QTL-seq:2019-08-30 22:59:15] start to run QTL-seq. [QTL-seq:2019-08-30 22:59:15] maximum number of threads which you can use is up to 72. [QTL-seq:2019-08-30 22:59:15] start to index reference fasta. [QTL-seq:2019-08-30 22:59:15] !!ERROR!! bwa index /home/user1/Test_dir/10_ref/Gmax_275_v2.0.fa >> /home/user1/Test_dir/log/bwa.log 2>&1
please check below:
[bwa_idx_build] fail to open file '/home/user1/Test_dir/10_ref/Gmax_275_v2.0.fa' : No such file or directory
