Hi All I have used MACS2 for the peak calling with no summit at the default settings and then used Diffbind to call differential peak calls. I am worried as I could not identify any differential peaks around the TSS but they lie somewhere else on the gene bodies. Is it is because I didn't use summit option while calling peaks using macs2 or differential peaks using DiffBind? Will the peak calling with summit option help or improve my calls? Any helpful suggestion is welcome.