Hi everyone,I am trying to generate a count matrix using abundance_estimates_to_matrix.pl script in Trinity,but I keep running into the same error that I failed to deal with.Any help/advice would be much appreciated! Thank you, Annika.
The script: /home/plc/softwares/trinityrnaseq-Trinity-v2.6.6/util/abundance_estimates_to_matrix.pl --est_method RSEM --gene_trans_map /home/Annika/chuli_data/trinity_denovo/Trinity.fasta.gene_trans_map --name_sample_by_basedir --out_prefix genes --quant_files 01RSEM/RSEM.genes.results 02RSEM/RSEM.genes.results The output was: Error, no TPM value specified for transcript [TRINITY_DN0_c0_g1_i1] of gene [TRINITY_DN0_c0_g1] for sample 01RSEM at /home/phlc/softwares/trinityrnaseq-Trinity-v2.6.6/util/abundance_estimates_to_matrix.pl line 322.
