RSEM error abundance_estimates_to_matrix.pl
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2.1 years ago
1716653041 • 0

Hi everyone,I am trying to generate a count matrix using abundance_estimates_to_matrix.pl script in Trinity,but I keep running into the same error that I failed to deal with.Any help/advice would be much appreciated! Thank you, Annika.

The script: /home/plc/softwares/trinityrnaseq-Trinity-v2.6.6/util/abundance_estimates_to_matrix.pl --est_method RSEM --gene_trans_map /home/Annika/chuli_data/trinity_denovo/Trinity.fasta.gene_trans_map --name_sample_by_basedir --out_prefix genes --quant_files 01RSEM/RSEM.genes.results 02RSEM/RSEM.genes.results The output was: Error, no TPM value specified for transcript [TRINITY_DN0_c0_g1_i1] of gene [TRINITY_DN0_c0_g1] for sample 01RSEM at /home/phlc/softwares/trinityrnaseq-Trinity-v2.6.6/util/abundance_estimates_to_matrix.pl line 322.

RNA-Seq abundance estimate rsem • 753 views
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