Question: How to extract gene signatures of multiple predefinded groups RNA-seq data.
gravatar for MatthewP
11 months ago by
MatthewP690 wrote:

Hello, I get DEGs of 2 groups sample use DESeq2. Also my samples can grouped into multiple groups according my experiment data. Now I need to filter genes in DEGs which will cluster my samples same as grouped by experiment data. Example, PCA use those genes expression data(DESeq2 normalized read counts) can seperate those different groups of samples.
I think this is an gene signature extraction with pre-definded group issue. I search a lot(blog, papers...) still don't know what's better/good method suits. Maybe it's hard to give me very detail method, but some direction or paper/blog example is also enough. I hope the method is simple both to understand(bad math skill) and perform. Thanks!
Note: I don't have big dataset, I only have 10 samples(3 groups totally).

gene signature rna-seq • 240 views
ADD COMMENTlink modified 10 months ago by Biostar ♦♦ 20 • written 11 months ago by MatthewP690
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