I am performing several analysis of differential expression (using edgeR). Reading edgeR manual I am not sure about BCV value that I should use. I am comparing libraries of the same species (non model species) and i have no biological replicates.
In the manual you say that "Typical values for the common BCV (square- root-dispersion) for datasets arising from well-controlled experiments are 0.4 for human data, 0.1 for data on genetically identical model organisms or 0.01 for technical replicates."
The species that I am studying is wheat . Can you please help me so I can decide which dispersion value to use.