Confusion with 2nd Read pair SRA download
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2.4 years ago
jaqx008 ▴ 110

Hello everone. I am trying to download an RNAseq data from ncbi. the data layout shows the reads are paired end. if I understand correctly, that means I will need to download two fastq files m1 and m2. However, if I open the link, i see 4 runs which I assume is the m1. How do I find the m2 please? I plan on using this with bowtie2. see ascession number below

SRX5972887

Thanks

SRA fastq PAIRED reads • 491 views
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2.4 years ago
GenoMax 111k

Post the accession number so people can offer informed advice. As recommended, get fastq data directly from EBI-ENA whenever possible.

These are paired-end data there are 4 submissions (each with a pair of files).

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Thank you I see it now on EBI-ENA

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Metadata looks the same for all 4 so it is possible that they submitted 4 lanes from a single NextSeq 550 flowcell as independent submissions.

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