Question: Alternate Splicing Analysis using rMATS for paired samples
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gravatar for ilovesuperheroes1993
17 months ago by
ilovesuperheroes199310 wrote:

I am using rMATS (version 4.0.2) to test for alternate splicing. I have 12 paired samples (12 replicates for normal and 12 replicates for diseased). Sample 1 (Normal) is paired with Sample 2 (Diseased) Sample 3 (Normal) is paired with Sample 4 (Diseased) ..and so on

It is mentioned that rMATS can test for both paired and unpaired replicates. However I do not find any options in rmats.py script to specify whether replicates are paired or unpaired. The only option I find is to create 2 lists, one for each condition, separated by comma.

So my list (for Normal) goes as sample1, sample3, sample5.. and my list (for Disease) goes as sample2, sample4, sample6..

How does rMATS know whether to test for paired replicates in this case?

ADD COMMENTlink written 17 months ago by ilovesuperheroes199310

I have found the answer. In the optional parameters there is the option --analysis Type P/U.

It seems that the above option was available for rMATS 3.4. This option is not available for the latest version. Why is this so?

ADD REPLYlink modified 17 months ago • written 17 months ago by ilovesuperheroes199310
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