Question: Setting the experimental design in a germline translatome analysis
0
gravatar for Assa Yeroslaviz
8 months ago by
Assa Yeroslaviz1.3k
Munich
Assa Yeroslaviz1.3k wrote:

I have a general question about an experiment we're planning. We would like to analyze how well various germline mRNA are being translated. For that we're planning to extract germline ribosome via Immunoprecipitation specifically for germline ribosomes.

For that i have two question. The first one is about the Input samples I should use. As I'm only interested in germline translational activity, I was wondering if it make sense to dissect the germlines for the input samples. The reason for that is that by dissecting them, I would make the input and the IPed samples more comparable (I think).

The second question is whether or not a depletion of the extract is needed in the purified ribosomal samples?

thanks

ADD COMMENTlink written 8 months ago by Assa Yeroslaviz1.3k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1082 users visited in the last hour