I am trying to figure out if my transcriptomic data (downloaded from ncbi) was made using strand or non strand method. This information was not provided alongside the download page. Is there a way I can do this using the fastq file?
You could quantify your data against a reference transcriptome with salmon and use the library type to automatic detection -l A. It will tell you then the most likely library type. Did you check the publication (if there is one) towards the method section / kit they used?