Off topic:Error information when running Hisat2 error correction for multiple mapping reads

Question

[hongliang.zhang]

Hi,

I did a Hisat2 error correction for multiple mapping reads after mapping. I have 9 libraries in total and the mapping rate is good (above 98 %). However, when I did the error correction, one library gave the following error:

Traceback (most recent call last):
  File "./toprintend1.py", line 16, in <module>
    start = int(splits[3].strip())
ValueError: invalid literal for int() with base 10: '149M'
[E::sam_parse1] SEQ and QUAL are of different length
[W::sam_read1] Parse error at line 114
[main_samview] truncated file.
errorCorrection.e8576242.9 (END)

All the other libraries ran well. Does anyone has any idea what happened here and how could I solve the problem? Thank you in advance!