Question: ChIP-seq differential binding analysis tools
0
gravatar for Jingyue
9 weeks ago by
Jingyue30
United States
Jingyue30 wrote:

Hello all,

Do you recommend to overlap two or more differential peak calling tools to get a more stringent/accurate differential peaks?

Thank you!

chip-seq diffbind csaw • 131 views
ADD COMMENTlink written 9 weeks ago by Jingyue30

I do not see why you would want to do that. It only makes things more complicated. If you want to be more stringent better lower the FDR cutoff from like 10% to 5 or 1% or test against a certain fold change, e.g. 1.5, using glmTreat in csaw (the actual function comes from edgeR). Combining different tools is tricky as one would need to ensure that normalization, filtering etc. is more or less identical to have a meaningful analysis.

ADD REPLYlink written 9 weeks ago by ATpoint26k
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