Importing .txt file and use Limma to normalize it...

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4.5 years ago

rozovski.uri • 0

I have microRNA array data that was generated in a platform that is no longer used. Basically these are unnormalized intensity data. The data is in .txt format (rows: geneID, Colum : conditions). I want to use limma to filter and normalize the data but I do notknow how to import into R this type of data.

Thanks, Uri

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ADD COMMENT • link 4.5 years ago by rozovski.uri • 0

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Please explain some more about the source of the data. For example: which platform / technology was used?

ADD REPLY • link 4.5 years ago by Kevin Blighe 87k

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This is a platform that is no longer exist but it is intensity data . It’s raw data (signal intensity) which I need to filter and normalize

ADD REPLY • link 4.5 years ago by rozovski.uri • 0

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Okay, but which platform?; and from where did you obtain the data? - these are important to know for the purpose of normalisation. Are the control probe intensities still in the data?

ADD REPLY • link 4.5 years ago by Kevin Blighe 87k

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