I wonder if anyone would like to share experience with Lexogen SENSE for de novo assembly? I am considering using the kit as it can deal with much lower RNA input (some of my samples have total RNA ca. 50 ng), but I have never tried the kit before. I am working with tree species with a genome (but not annotation) available. I am thinking about doing genome-guided de novo assembly.
Thanks in advance! Any comments and suggestions are welcome!