Question: Infer Experiment strand information interpretation
0
gravatar for dnzkursun
7 months ago by
dnzkursun10
dnzkursun10 wrote:

Hi, Below is the result of RSeQC Infer Experiment. Sorry for the such a naive question but I want to make sure if the library type is reverse stranded (aka fr-firststrand, RF) before moving on the analysis. This is PairEnd Data

Fraction of reads failed to determine: 0.0087
Fraction of reads explained by "1++,1--,2+-,2-+": 0.0184
Fraction of reads explained by "1+-,1-+,2++,2--": 0.9729
rseqc rna-seq stranded • 318 views
ADD COMMENTlink modified 5 months ago by Biostar ♦♦ 20 • written 7 months ago by dnzkursun10

See A: Infer-experiment.py, is strand-specific?

ADD REPLYlink written 7 months ago by genomax84k

Then, it is fr-firststrand. Thank you for the link.

ADD REPLYlink written 7 months ago by dnzkursun10
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 895 users visited in the last hour